ARG64285
anti-COX4 antibody
anti-COX4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes COX4 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize COX4I1 (GeneID:1327; MW: 19.6kDa) & COX4I2 (GeneID:84701, MW:19.6kDa). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | COX4 |
| 抗原物种 | Human |
| 抗原 | C-QGLASKWDYEKNE |
| 偶联标记 | Un-conjugated |
| 別名 | Cytochrome c oxidase polypeptide IV; COX4; COX4-1; COXIV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4I1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P13073 Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
|---|---|
| 基因名称 | COX4I1 |
| 全名 | cytochrome c oxidase subunit IV isoform 1 |
| 背景介绍 | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| 生物功能 | COX4 protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. [UniProt] |
| 研究领域 | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody; Loading Control antibody for Cytoplasmic Fractions; Cytochrome-C fractionation Study antibody; Mitochondrial Marker antibody |
| 预测分子量 | 20 kDa |
检测图片 (3) Click the Picture to Zoom In
-
ARG64285 anti-COX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64285 anti-COX4 antibody at 2.5 µg/ml dilution, followed by AP-staining.
-
ARG64285 anti-COX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64285 anti-COX4 antibody at 2.5 µg/ml dilution followed by AP-staining.
-
ARG64285 anti-COX4 antibody WB image
Western blot: 35 µg of Human muscle lysate stained with ARG64285 anti-COX4 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
