ARG57135
anti-COTL1 antibody [1D6]
anti-COTL1 antibody [1D6] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1D6] recognizes COTL1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1D6 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | COTL1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-142 of Human COTL1 |
| 偶联标记 | Un-conjugated |
| 別名 | Coactosin-like protein; CLP |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COTL1 |
| 全名 | coactosin-like F-actin binding protein 1 |
| 背景介绍 | This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008] |
| 生物功能 | Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis. [UniProt] |
| 预测分子量 | 16 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG57135 anti-COTL1 antibody [1D6] FACS image
Flow Cytometry: HeLa cell line stained with ARG57135 anti-COTL1 antibody [1D6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57135 anti-COTL1 antibody [1D6] WB image
Western blot: 40 µg of SW480 cell lysate stained with ARG57135 anti-COTL1 antibody [1D6] at 1:1000.
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ARG57135 anti-COTL1 antibody [1D6] WB image
Western blot: 20 µg of 1) 293T, and 2) COTL1 transfected 293T cell lysate stained with ARG57135 anti-COTL1 antibody [1D6] at 1:2000.
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ARG57135 anti-COTL1 antibody [1D6] WB image
Western blot: 40 µg of 1) PC3, 2) A549, 3) U87-MG, and 4) HeLa cell lysates stained with ARG57135 anti-COTL1 antibody [1D6] at 1:500.
