ARG40149
anti-CORO1B phospho (Ser2) antibody
anti-CORO1B phospho (Ser2) antibody for ICC/IF,Western blot and Human,Rabbit
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CORO1B phospho (Ser2) |
|---|---|
| 反应物种 | Hu, Rb |
| 预测物种 | Ms, Rat |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody detects a ~60 kDa band corresponding to Coronin-1B on SDS-PAGE immunoblots of A431 or HeLa cells treated with Calyculin A. This band is not observed in control cells and the band observed in Calyculin A-treated cells can be removed with lambda phosphatase treatment. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CORO1B |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide (coupled to carrier protein) around Ser2 of Human CORO1B. This sequence has high homology to the conserved site in Rat and Mouse CORO1B and in CORO1A. However, this sequence has low homology to the conserved region in other coronins. |
| 偶联标记 | Un-conjugated |
| 別名 | Coronin-1B; Coronin-2; CORONIN-2 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
GeneID: 100009415 Rabbit CORO1B |
|---|---|
| 基因名称 | CORO1B |
| 全名 | coronin, actin binding protein, 1B |
| 背景介绍 | Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Note=Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40149 anti-CORO1B phospho (Ser2) antibody WB image
Western blot: A431 cells treated with Calyculin A (100 nM) for 30 min (left) before treatment with lambda phosphatase (right). The blots were stained with ARG40149 anti-CORO1B phospho (Ser2) antibody.
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ARG40149 anti-CORO1B phospho (Ser2) antibody ICC/IF image
Immunofluorescence: Rabbit spleen fibroblasts treated with Calyculin A. The cells were stained with ARG40149 anti-CORO1B phospho (Ser2) antibody (red). The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.
