ARG40148
anti-CORO1B antibody
anti-CORO1B antibody for ICC/IF,Western blot and Human,Mouse,Rabbit
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CORO1B |
|---|---|
| 反应物种 | Hu, Ms, Rb |
| 预测物种 | Rat |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody detects a ~60 kDa band corresponding to Coronin-1B on SDS-PAGE immunoblots of A431, HeLa, Mouse brain and Rabbit spleen fibroblasts. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CORO1B |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (coupled to carrier protein) around the C-terminus of Human CORO1B. This sequence has high homology to the conserved site in Rat and Mouse CORO1B, and less than 50% homology to similar regions in other coronins. |
| 偶联标记 | Un-conjugated |
| 別名 | Coronin-1B; Coronin-2; CORONIN-2 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 50% Glycerol and 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CORO1B |
| 全名 | coronin, actin binding protein, 1B |
| 背景介绍 | Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton. Note=Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40148 anti-CORO1B antibody WB image
Western blot: A431 cells treated with Calyculin A (100 nM) for 30 min (left) before treatment with lambda phosphatase (right). The blots were stained with ARG40148 anti-CORO1B antibody.
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ARG40148 anti-CORO1B antibody ICC/IF image
Immunofluorescence: Rabbit spleen fibroblasts treated with Calyculin A. The cells were stained with ARG40148 anti-CORO1B antibody (red). The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.
