ARG45646
anti-COPS5 / JAB1 antibody
anti-COPS5 / JAB1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes COPS5 / JAB1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | COPS5 / JAB1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human COPS5 / JAB1 . |
| 偶联标记 | Un-conjugated |
| 別名 | COPS5; COP9 signalosome subunit 5; MOV-34; Jun activation domain-binding protein 1; EC 3.4.-.-; JAB1; Signalosome subunit 5; SGN5; COP9 signalosome complex subunit 5; CSN5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 实际分子量 | 38 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O35864 Mouse COP9 signalosome complex subunit 5 Swiss-port # Q92905 Human COP9 signalosome complex subunit 5 |
|---|---|
| 基因名称 | COPS5 |
| 全名 | COP9 signalosome subunit 5 |
| 背景介绍 | The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq, Jul 2008] |
| 生物功能 | Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Note=Nuclear localization is diminished in the presence of IFIT3. [UniProt] |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Acetylation. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG45646 anti-COPS5 / JAB1 antibody IHC-P image
Immunohistochemistry: Human mammary cancer stained with ARG45646 anti-COPS5 / JAB1 antibody at 2 μg/ml dilution.
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ARG45646 anti-COPS5 / JAB1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45646 anti-COPS5 / JAB1 antibody at 5 μg/ml dilution.
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ARG45646 anti-COPS5 / JAB1 antibody WB image
Western blot: A549 stained with ARG45646 anti-COPS5 / JAB1 antibody at 0.5 μg/ml dilution.
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ARG45646 anti-COPS5 / JAB1 antibody FACS image
Flow Cytometry: U2OS stained with ARG45646 anti-COPS5 / JAB1 antibody at 1 µg/10^6 cells dilution.
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ARG45646 anti-COPS5 / JAB1 antibody IHC-P image
Immunohistochemistry: Rat testis stained with ARG45646 anti-COPS5 / JAB1 antibody at 2 μg/ml dilution.
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ARG45646 anti-COPS5 / JAB1 antibody WB image
Western blot: Rat brain stained with ARG45646 anti-COPS5 / JAB1 antibody at 0.5 μg/ml dilution.
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ARG45646 anti-COPS5 / JAB1 antibody IHC-P image
Immunohistochemistry: Mouse testis stained with ARG45646 anti-COPS5 / JAB1 antibody at 2 μg/ml dilution.
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ARG45646 anti-COPS5 / JAB1 antibody WB image
Western blot: Mouse brain stained with ARG45646 anti-COPS5 / JAB1 antibody at 0.5 μg/ml dilution.
