ARG67029
anti-COL4A3 antibody [SQab30316]
anti-COL4A3 antibody [SQab30316] for IHC-Formalin-fixed paraffin-embedded sections and Human
概述
| 产品描述 | Recombinant rabbit Monoclonal antibody [SQab30316] recognizes COL4A3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab30316 |
| 同位型 | IgG |
| 靶点名称 | COL4A3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human COL4A3. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Collagen alpha-3(IV) chain |
| 別名 | COL4A3, Collagen Type IV Alpha 3 Chain, Collagen, Type IV, Alpha 3 (Goodpasture Antigen), Collagen Alpha-3(IV) Chain, Tumstatin, Collagen IV, Alpha-3 Polypeptide, Goodpasture Antigen, ATS2, ATS3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 37 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | Human kidney |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05%BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | COL4A3 |
|---|---|
| 全名 | Collagen Type IV Alpha 3 Chain |
| 背景介绍 | Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010] |
| 生物功能 | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms. [Uniprot] |
| 细胞定位 | Basement membrane, Extracellular matrix, Secreted |
| 预测分子量 | 162 kDa |
| 翻译后修饰 | Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.Isoform 2 contains an additional N-linked glycosylation site.Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. [Uniprot] |
检测图片 (1) Click the Picture to Zoom In
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ARG67029 anti-COL4A3 antibody [SQab30316] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded human kidney stained with ARG67029 anti-COL4A3 antibody [SQab30316].
