anti-COL4A3 antibody [SQab30316]

anti-COL4A3 antibody [SQab30316] for IHC-Formalin-fixed paraffin-embedded sections and Human


产品描述 Recombinant rabbit Monoclonal antibody [SQab30316] recognizes COL4A3
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab30316
同位型 IgG
靶点名称 COL4A3
抗原物种 Human
抗原 Recombinant protein of Human COL4A3.
偶联标记 Un-conjugated
Protein Full name Collagen alpha-3(IV) chain
別名 COL4A3, Collagen Type IV Alpha 3 Chain, Collagen, Type IV, Alpha 3 (Goodpasture Antigen), Collagen Alpha-3(IV) Chain, Tumstatin, Collagen IV, Alpha-3 Polypeptide, Goodpasture Antigen, ATS2, ATS3


应用 推荐稀释比
IHC-P1:50 - 1:100
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human kidney


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05%BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 1285 Human COL4A3

Swiss-port # Q01955 Human Collagen alpha-3(IV) chain

基因名称 COL4A3
全名 Collagen Type IV Alpha 3 Chain
背景介绍 Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]
生物功能 Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms. [Uniprot]
细胞定位 Basement membrane, Extracellular matrix, Secreted
预测分子量 162 kDa
翻译后修饰 Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.Isoform 2 contains an additional N-linked glycosylation site.Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues. [Uniprot]

检测图片 (1) Click the Picture to Zoom In

  • ARG67029 anti-COL4A3 antibody [SQab30316] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded human kidney stained with ARG67029 anti-COL4A3 antibody [SQab30316].