ARG56713

anti-CNTF antibody

anti-CNTF antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes CNTF
反应物种 Hu
应用 ELISA, IHC-P, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CNTF
抗原物种 Human
抗原 E.coli derived Recombinant Human CNTF.
(AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTQSYVKH QGLNKNINLD SADGMPVAST DQWSQLTQAQ RLQQNLQAYR TFHVLLARLL QDQQVHFTPT QGDFHQAIHT LLLQVAAFAY QIQQLMILLQ YKIPRNQADG MPINVGDGGL FQKKLWGLKV LQQLSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM)
偶联标记 Un-conjugated
別名 HCNTF; CNTF; Ciliary neurotrophic factor

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56822 as a detection antibody
IHC-P0.25 µg/ml
Neut7.0 - 11.0 µg/ml (To yield [ND50] of the biological activity of Human CNTF (1.0 µg/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1270 Human CNTF

Swiss-port # P26441 Human Ciliary neurotrophic factor

基因名称 CNTF
全名 ciliary neurotrophic factor
背景介绍 The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]
生物功能 CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. [UniProt]
预测分子量 23 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG56713 anti-CNTF antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56713 anti-CNTF antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG56713 anti-CNTF antibody WB image

    Western blot: 250 - 0.24 ng of Human CNTF stained with ARG56713 anti-CNTF antibody, under reducing conditions.

  • ARG56713 anti-CNTF antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56713 anti-CNTF antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG56713 anti-CNTF antibody WB image

    Western blot: 250 - 0.24 ng of Human CNTF stained with ARG56713 anti-CNTF antibody, under non-reducing conditions.

  • ARG56713 anti-CNTF antibody standard curve image

    Sandwich ELISA: ARG56713 anti-CNTF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56822 anti-CNTF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.