ARG64963
anti-CLIP1 / CLIP170 antibody
anti-CLIP1 / CLIP170 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CLIP1 / CLIP170 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Dog, Pig |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CLIP1 / CLIP170 |
| 抗原物种 | Human |
| 抗原 | C-PHSTHHGSRGEERP |
| 偶联标记 | Un-conjugated |
| 別名 | CLIP170; RSN; CLIP; CLIP-170; Restin; Cytoplasmic linker protein 1; CAP-Gly domain-containing linker protein 1; CYLN1; Cytoplasmic linker protein 170 alpha-2; Reed-Sternberg intermediate filament-associated protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30622 Human CAP-Gly domain-containing linker protein 1 |
|---|---|
| 背景介绍 | The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 162 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons (By similarity). Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules (PubMed:12231510). |
检测图片 (3) Click the Picture to Zoom In
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ARG64963 anti-CLIP1 / CLIP170 antibody WB image
Western blot: 35 µg of Human Breast Cancer lysate stained with ARG64963 anti-CLIP1 / CLIP170 antibody at 1 µg/ml dilution.
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ARG64963 anti-CLIP1 / CLIP170 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64963 anti-CLIP1 / CLIP170 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64963 anti-CLIP1 / CLIP170 antibody IHC image
Immunohistochemistry: paraffin-embedded Human Thyroid Gland (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64963 anti-CLIP1 / CLIP170 antibody at 2.5 µg/ml dilution, followed by AP-staining.
