ARG54238
anti-CLIC5a antibody [CLIC5-02] (PE)
anti-CLIC5a antibody [CLIC5-02] (PE) for Flow cytometry and Human
Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [CLIC5-02] recognizes CLIC5a |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone CLIC5-02 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Crossreactivity with CLIC5b was not determined. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CLIC5-02 |
| 同位型 | IgG2b |
| 靶点名称 | CLIC5a |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a |
| 偶联标记 | PE |
| 別名 | Chloride intracellular channel protein 5; DFNB102; DFNB103; MST130; MSTP130 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size-exclusion chromatography. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NZA1 Human Chloride intracellular channel protein 5 |
|---|---|
| 基因名称 | CLIC5 |
| 全名 | chloride intracellular channel 5 |
| 背景介绍 | CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures. |
| 生物功能 | Required for normal hearing (PubMed:24781754). It is necessary for the formation of stereocilia in the inner ear and normal development of the organ of Corti (By similarity). Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. May play a role in the regulation of transepithelial ion absorption and secretion. Is required for the development and/or maintenance of the proper glomerular endothelial cell and podocyte architecture (PubMed:15184393, PubMed:18028448, PubMed:20335315). [UniProt] |
| 研究领域 | Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 47 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG54238 anti-CLIC5a antibody [CLIC5-02] (PE) FACS image
Flow Cytometry: Separation of unstained (black-dashed) and stained (red-filled) CLIC5 transfected HEK293 cells. Cells were stained with ARG54238 anti-CLIC5a antibody [CLIC5-02] (PE) at 3 µg/ml dilution.
