ARG64264

anti-CLIC4 antibody

anti-CLIC4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes CLIC4
反应物种 Hu, Ms
预测物种 Dog, Rat
应用 ICC/IF, IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 CLIC4
抗原物种 Human
抗原 NGLKEEDKEPLIE-C
偶联标记 Un-conjugated
別名 Chloride intracellular channel protein 4; MTCLIC; H1; Intracellular chloride ion channel protein p64H1; huH1; p64H1; CLIC4L

应用说明

应用建议
应用 推荐稀释比
ICC/IF5 µg/ml
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 25932 Human CLIC4

GeneID: 29876 Mouse CLIC4

Swiss-port # Q9QYB1 Mouse Chloride intracellular channel protein 4

Swiss-port # Q9Y696 Human Chloride intracellular channel protein 4

背景介绍 Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]
研究领域 Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 29 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG64264 anti-CLIC4 antibody WB image

    Western Blot: human kidney lysate (35 µg protein in RIPA buffer) stained with ARG64264 anti-CLIC4 antibody at 0.1 µg/ml dilution.

  • ARG64264 anti-CLIC4 antibody ICC/IF image

    Immunofluorescence: MIN6 cells transiently expressing Mouse Clic4. Cells were stained with ARG64264 anti-CLIC4 antibody at 5 µg/ml dilution. Hoechst (blue) for nuclear staining.

  • ARG64264 anti-CLIC4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64264 anti-CLIC4 antibody at 5 µg/ml dilution followed by AP-staining.