ARG63612

anti-CLDN14 / Claudin 14 antibody

anti-CLDN14 / Claudin 14 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Neuroscience antibody; Signaling Transduction antibody
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anti-CLDN14 / Claudin 14 antibody Goat Polyclonal antibody recognizes CLDN14 / Claudin 14 ARG63612 Arigo biolaboratories
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anti-CLDN14 / Claudin 14 antibody

概述

产品描述 Goat Polyclonal antibody recognizes CLDN14 / Claudin 14
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog, Pig
应用 IHC-P, WB
特异性 Reported variants represent identical protein (NP_036262.1; NP_652763.1; NP_001139551.1; NP_001139550.1 and NP_001139549.1).
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 CLDN14 / Claudin 14
抗原物种 Human
抗原 C-SATHSGYRLNDYV
偶联标记 Un-conjugated
別名 DFNB29; Claudin-14

应用说明

应用建议
应用 推荐稀释比
IHC-PAssay - dependent
WB0.3 - 1 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
预测分子量 26 kDa

属性

形式 Liquid
纯化 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 23562 Human CLDN14

Swiss-port # O95500 Human Claudin-14

背景介绍 Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. It is also reported that four synonymous variants in this gene are associated with kidney stones and reduced bone mineral density. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010]
研究领域 Neuroscience antibody; Signaling Transduction antibody

检测图片 (2) Click the Picture to Zoom In

  • ARG63612 anti-CLDN14 / Claudin 14 antibody WB image

    Western blot: Human liver lysate (35 µg protein in RIPA buffer) stained with ARG63612 anti-CLDN14 / Claudin 14 antibody at 0.3 µg/ml dilution.

  • ARG63612 anti-CLDN14 / Claudin 14 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63612 anti-CLDN14 / Claudin 14 antibody at 5 µg/ml dilution followed by AP-staining.

客户反馈

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anti-CLDN14 / Claudin 14 antibody

Application:WB

Sample:Mouse liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC