ARG45390

anti-CLCN3 antibody

anti-CLCN3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes CLCN3
反应物种 Hu, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CLCN3
抗原物种 Human
抗原 Recombinant protein containing to human CLCN3.
偶联标记 Un-conjugated
別名 CLCN3; Chloride Voltage-Gated Channel 3; ClC-3; CLC3; Chloride Channel, Voltage-Sensitive 3; H(+)/Cl(-) Exchange Transporter 3; Chloride Channel Protein 3; Chloride Transporter ClC-3; Chloride Channel 3; NEDHYBA; NEDSBA

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 170 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1182 Human CLCN3

GeneID: 84360 Rat CLCN3

Swiss-port # P51790 Human H(+)/Cl(-) exchange transporter 3

Swiss-port # P51792 Rat H(+)/Cl(-) exchange transporter 3

基因名称 CLCN3
全名 Chloride Voltage-Gated Channel 3
背景介绍 This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
生物功能 Isoform 1_x000D_ Strongly outwardly rectifying, electrogenic H+/Cl-exchanger which mediates the exchange of chloride ions against protons ._x000D_ The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons.
Strongly outwardly rectifying, electrogenic H+/Cl-exchanger which mediates the exchange of chloride ions against protons. [UniProt]
细胞定位 Cell membrane; Cell projection; Endosome; Golgi apparatus; Lysosome; Membrane. [UniProt]
预测分子量 91 kDa
翻译后修饰 Glycoprotein. [UniProt]

检测图片 (5) Click the Picture to Zoom In

  • ARG45390 anti-CLCN3 antibody IHC-P image

    Immunohistochemistry: Human prostatic cancer stained with ARG45390 anti-CLCN3 antibody at 2 μg/ml dilution.

  • ARG45390 anti-CLCN3 antibody ICC/IF image

    Immunofluorescence: SiHa stained with ARG45390 anti-CLCN3 antibody at 5 μg/ml dilution.

  • ARG45390 anti-CLCN3 antibody WB image

    Western blot: Hela and K562 stained with ARG45390 anti-CLCN3 antibody at 0.5 μg/ml dilution.

  • ARG45390 anti-CLCN3 antibody FACS image

    Flow Cytometry: JK stained with ARG45390 anti-CLCN3 antibody at 1 µg/10^6 cells dilution.

  • ARG45390 anti-CLCN3 antibody IHC-P image

    Immunohistochemistry: Rat kidney stained with ARG45390 anti-CLCN3 antibody at 2 μg/ml dilution.