ARG42039
anti-CLASP1 antibody [KT66]
anti-CLASP1 antibody [KT66] for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rat Monoclonal antibody [KT66] recognizes CLASP1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Chk |
| 应用 | ICC/IF, WB |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | KT66 |
| 同位型 | IgG2a |
| 靶点名称 | CLASP1 |
| 抗原物种 | Mouse |
| 抗原 | GST fusion protein around the C-terminus of Mouse CLASP1. |
| 偶联标记 | Un-conjugated |
| 別名 | Multiple asters homolog 1; Protein Orbit homolog 1; MAST1; CLIP-associating protein 1; hOrbit1; Cytoplasmic linker-associated protein 1 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 161 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.05% Sodium azide. |
| 抗菌剂 | 0.05% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CLASP1 |
| 全名 | cytoplasmic linker associated protein 1 |
| 背景介绍 | CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton, spindle, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Golgi apparatus, trans-Golgi network. Note=Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. [UniProt] |
| 预测分子量 | 169 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42039 anti-CLASP1 antibody [KT66] WB image
Western blot: Mouse brain and PC-12 cell lysates stained with ARG42039 anti-CLASP1 antibody [KT66] at 1:500 dilution.
