ARG57033
anti-CKMT1A antibody [17A2]
anti-CKMT1A antibody [17A2] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [17A2] recognizes CKMT1A |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 17A2 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | CKMT1A |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 40-417 of Human CKMT1A. |
| 偶联标记 | Un-conjugated |
| 別名 | CKMT1; U-MtCK; mia-CK |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P12532 Human Creatine kinase U-type, mitochondrial |
|---|---|
| 基因名称 | CKMT1A |
| 全名 | creatine kinase, mitochondrial 1A |
| 背景介绍 | Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [UniProt] |
| 预测分子量 | 47 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57033 anti-CKMT1A antibody [17A2] FACS image
Flow Cytometry: HeLa cell line stained with ARG57033 anti-CKMT1A antibody [17A2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57033 anti-CKMT1A antibody [17A2] WB image
Western blot: 40 µg of 1) 293T cell lysate, and 2) HeLa cell lysate stained with ARG57033 anti-CKMT1A antibody [17A2] at 1:1000.
