ARG43141
anti-CISD1 / mitoNEET antibody
anti-CISD1 / mitoNEET antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CISD1 / mitoNEET |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mk |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CISD1 / mitoNEET |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to Y28-E93 of Human CISD1 / mitoNEET. |
| 偶联标记 | Un-conjugated |
| 別名 | MDS029; mitoNEET; C10orf70; CDGSH iron-sulfur domain-containing protein 1; MitoNEET; ZCD1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 15 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CISD1 |
| 全名 | CDGSH iron sulfur domain 1 |
| 背景介绍 | This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012] |
| 生物功能 | Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions. [UniProt] |
| 细胞定位 | Mitochondrion outer membrane; Single-pass type III membrane protein. [UniProt] |
| 预测分子量 | 12 kDa |
| 翻译后修饰 | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG43141 anti-CISD1 / mitoNEET antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43141 anti-CISD1 / mitoNEET antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43141 anti-CISD1 / mitoNEET antibody WB image
Western blot: 50 µg of sample under reducing conditions. SW620, Monkey heart, Rat heart, Rat kidney and Mouse heart lysates stained with ARG43141 anti-CISD1 / mitoNEET antibody at 0.25 µg/ml dilution, overnight at 4°C.
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ARG43141 anti-CISD1 / mitoNEET antibody FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG43141 anti-CISD1 / mitoNEET antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG43141 anti-CISD1 / mitoNEET antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43141 anti-CISD1 / mitoNEET antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43141 anti-CISD1 / mitoNEET antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43141 anti-CISD1 / mitoNEET antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG43141 anti-CISD1 / mitoNEET antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43141 anti-CISD1 / mitoNEET antibody at 2 µg/ml dilution, overnight at 4°C.
