ARG57013
anti-CISD1 / mitoNEET antibody [1A8]
anti-CISD1 / mitoNEET antibody [1A8] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1A8] recognizes CISD1 / mitoNEET |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1A8 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CISD1 / mitoNEET |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 32-108 of Human CISD1 / mitoNEET. |
| 偶联标记 | Un-conjugated |
| 別名 | MDS029; mitoNEET; C10orf70; CDGSH iron-sulfur domain-containing protein 1; MitoNEET; ZCD1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NZ45 Human CDGSH iron-sulfur domain-containing protein 1 |
|---|---|
| 基因名称 | CISD1 |
| 全名 | CDGSH iron sulfur domain 1 |
| 背景介绍 | This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012] |
| 生物功能 | Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions. [UniProt] |
| 预测分子量 | 12 kDa |
| 翻译后修饰 | Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30. |
检测图片 (3) Click the Picture to Zoom In
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ARG57013 anti-CISD1 / mitoNEET antibody [1A8] FACS image
Flow Cytometry: THP-1 cell line stained with ARG57013 anti-CISD1 / mitoNEET antibody [1A8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57013 anti-CISD1 / mitoNEET antibody [1A8] WB image
Western blot: 40 µg of Mouse liver lysate stained with ARG57013 anti-CISD1 / mitoNEET antibody [1A8] at 1:1000.
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ARG57013 anti-CISD1 / mitoNEET antibody [1A8] WB image
Western blot: 40 µg of 1) K562 cell lysate, 2) MCF7 cell lysate stained with ARG57013 anti-CISD1 / mitoNEET antibody [1A8] at 1:1000.
