ARG58428
anti-CHRNA5 antibody
anti-CHRNA5 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CHRNA5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CHRNA5 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence at the N-terminus of Human CHRNA5 (44-76aa AKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKF), different from the related Mouse sequence by five amino acids, and from the related Rat sequence by four amino acids. |
| 偶联标记 | Un-conjugated |
| 別名 | LNCR2; Neuronal acetylcholine receptor subunit alpha-5 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30532 Human Neuronal acetylcholine receptor subunit alpha-5 Swiss-port # Q2MKA5 Mouse Neuronal acetylcholine receptor subunit alpha-5 |
|---|---|
| 基因名称 | CHRNA5 |
| 全名 | cholinergic receptor, nicotinic, alpha 5 (neuronal) |
| 背景介绍 | The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010] |
| 生物功能 | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. [UniProt] |
| 细胞定位 | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 53 kDa |
检测图片 (9) Click the Picture to Zoom In
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ARG58428 anti-CHRNA5 antibody FACS image
Flow Cytometry: U251 cells were blocked with 10% normal goat serum, and then stained with ARG58428 anti-CHRNA5 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58428 anti-CHRNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58428 anti-CHRNA5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58428 anti-CHRNA5 antibody WB image
Western blot: 50 µg of Rat skeletal muscle and HepG2 whole cell lysate stained with ARG58428 anti-CHRNA5 antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.
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ARG58428 anti-CHRNA5 antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum, and then stained with ARG58428 anti-CHRNA5 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58428 anti-CHRNA5 antibody FACS image
Flow Cytometry: MCF-7 cells were blocked with 10% normal goat serum, and then stained with ARG58428 anti-CHRNA5 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58428 anti-CHRNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse cardiac muscle tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58428 anti-CHRNA5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58428 anti-CHRNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58428 anti-CHRNA5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58428 anti-CHRNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat cardiac muscle tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58428 anti-CHRNA5 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG58428 anti-CHRNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostatic cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58428 anti-CHRNA5 antibody at 1 µg/ml dilution, overnight at 4°C.
