ARG58685
anti-CHRNA3 antibody
anti-CHRNA3 antibody for Flow cytometry,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CHRNA3 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CHRNA3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human CHRNA3 (DAVLSLSALSPEIKEAIQSVKYIAENMKAQNEAKEIQD). |
| 偶联标记 | Un-conjugated |
| 別名 | LNCR2; NACHRA3; Neuronal acetylcholine receptor subunit alpha-3; PAOD2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P32297 Human Neuronal acetylcholine receptor subunit alpha-3 Swiss-port # Q8R4G9 Mouse Neuronal acetylcholine receptor subunit alpha-3 |
|---|---|
| 基因名称 | CHRNA3 |
| 全名 | cholinergic receptor, nicotinic, alpha 3 (neuronal) |
| 背景介绍 | This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
| 生物功能 | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. [UniProt] |
| 细胞定位 | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 57 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG58685 anti-CHRNA3 antibody FACS image
Flow Cytometry: U251 cells were blocked with 10% normal goat serum, and then stained with ARG58685 anti-CHRNA3 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG58685 anti-CHRNA3 antibody WB image
Western blot: 50 µg of HeLa whole cell lysates, MDA-MB-453 whole cell lysates, Jurkat whole cell lysates, HepG2 whole cell lysates, SK-OV-3 whole cell lysates, PANC-1 whole cell lysates and Mouse thymus stained with ARG58685 anti-CHRNA3 antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.
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ARG58685 anti-CHRNA3 antibody FACS image
Flow Cytometry: U-87 cells were blocked with 10% normal goat serum, and then stained with ARG58685 anti-CHRNA3 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
