ARG66466

anti-CHRAC17 antibody

anti-CHRAC17 antibody for ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes CHRAC17
反应物种 Hu
预测物种 Ms, Rat
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CHRAC17
抗原物种 Human
抗原 Recombinant full length protein of Human CHRAC17.
偶联标记 Un-conjugated
別名 DNA polymerase II subunit 3; CHRAC-17; HuCHRAC17; Chromatin accessibility complex 17 kDa protein; YBL1; AsTP; DNA polymerase epsilon subunit p17; Arsenic-transactivated protein; p17; CHARAC17; CHRAC17; EC 2.7.7.7; DNA polymerase epsilon subunit 3

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:100
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 17 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
抗菌剂 0.01% Sodium azide
稳定剂 30% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 54107 Human POLE3

Swiss-port # Q9NRF9 Human DNA polymerase epsilon subunit 3

基因名称 POLE3
全名 polymerase (DNA directed), epsilon 3, accessory subunit
背景介绍 POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
生物功能 Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 17 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG66466 anti-CHRAC17 antibody ICC/IF image

    Immunofluorescence: Formalin-fixed U2OS cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66466 anti-CHRAC17 antibody (red) in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber.

  • ARG66466 anti-CHRAC17 antibody WB image

    Western blot: MCF7 whole cell lysate stained with ARG66466 anti-CHRAC17 antibody.