ARG45801

anti-CHP2 antibody

anti-CHP2 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes CHP2
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CHP2
抗原物种 Human
抗原 Recombinant protein containing to human CHP2.
偶联标记 Un-conjugated
別名 CHP2; Calcineurin Like EF-Hand Protein 2; Hepatocellular Carcinoma-Associated Antigen 520; Calcineurin B Homologous Protein 2; Hepatocellular Carcinoma Antigen Gene 520; HCA520

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 22 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 63928 Human CHP2

Swiss-port # O43745 Human Calcineurin B homologous protein 2

基因名称 CHP2
全名 Calcineurin Like EF-Hand Protein 2
背景介绍 This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013]
生物功能 Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus. [UniProt]
细胞定位 Cell membrane; Cytoplasm; Membrane; Nucleus. [UniProt]
预测分子量 22 kDa
翻译后修饰 Lipoprotein; Myristate; Phosphoprotein. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45801 anti-CHP2 antibody ICC/IF image

    Immunofluorescence: Hela stained with ARG45801 anti-CHP2 antibody at 5 μg/ml dilution.

  • ARG45801 anti-CHP2 antibody WB image

    Western blot: Hela stained with ARG45801 anti-CHP2 antibody at 0.5 μg/ml dilution.

  • ARG45801 anti-CHP2 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG45801 anti-CHP2 antibody at 1 µg/10^6 cells dilution.