ARG40721
anti-CHMP5 antibody
anti-CHMP5 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CHMP5 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CHMP5 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to aa. 1-219 of Human CHMP5. |
| 偶联标记 | Un-conjugated |
| 別名 | hVps60; Charged multivesicular body protein 5; SNF7 domain-containing protein 2; HSPC177; CGI-34; Vps60; PNAS-2; Chromatin-modifying protein 5; Vacuolar protein sorting-associated protein 60; SNF7DC2; C9orf83 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 实际分子量 | 25 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Caprylic acid ammonium sulfate precipitation. |
| 缓冲液 | 0.01M PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol. |
| 抗菌剂 | 0.03% Proclin 300 |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NZZ3 Human Charged multivesicular body protein 5 |
|---|---|
| 基因名称 | CHMP5 |
| 全名 | charged multivesicular body protein 5 |
| 背景介绍 | CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. [UniProt] |
| 细胞定位 | Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein. Note=Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. [UniProt] |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | ISGylated. Isgylation inhibits its interaction with VTA1. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40721 anti-CHMP5 antibody WB image
Western blot: 293T and K562 whole cell lysates stained with ARG40721 anti-CHMP5 antibody at 2 µg/ml dilution.
