ARG63479
anti-CHFR antibody
anti-CHFR antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CHFR |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Pig |
| 应用 | IHC-P |
| 特异性 | This antibody is expected to recognise all reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CHFR |
| 抗原物种 | Human |
| 抗原 | KFNHICEQTRFKN |
| 偶联标记 | Un-conjugated |
| 別名 | EC 6.3.2.-; RNF196; E3 ubiquitin-protein ligase CHFR; RNF116; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 73 kDa |
| 翻译后修饰 | Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1. Autoubiquitinated; may regulate its cellular level. Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity. |
检测图片 (1) Click the Picture to Zoom In
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ARG63479 anti-CHFR antibody IHC-P image
Immunohistochemistry: paraffin embedded human brain (cerebellum). (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63479 anti-CHFR antibody at 2.5 µg/ml dilution followed by AP-staining.
