ARG54652
anti-CHEK2 antibody
anti-CHEK2 antibody for ELISA,ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CHEK2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CHEK2 |
| 抗原 | Synthetic peptide within the first 50 aa of Human Chk2 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Hucds1; PP1425; CDS1; Serine/threonine-protein kinase Chk2; Checkpoint kinase 2; CHK2; hCds1; RAD53; HuCds1; LFS2; CHK2 checkpoint homolog; EC 2.7.11.1; Cds1 homolog |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat Cell Lysate | ||||||||
| 实际分子量 | 60 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O96017 Human Serine/threonine-protein kinase Chk2 Swiss-port # Q9Z265 Mouse Serine/threonine-protein kinase Chk2 |
|---|---|
| 基因名称 | CHEK2 |
| 全名 | checkpoint kinase 2 |
| 背景介绍 | Chk2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer. | |
| 研究领域 | Cancer antibody; Gene Regulation antibody |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4. Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination. |
检测图片 (2) Click the Picture to Zoom In
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ARG54652 anti-CHEK2 antibody ICC/IF image
Immunocytochemistry: Jurkat cells stained with ARG54652 anti-CHEK2 antibody at 1 μg/ml.
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ARG54652 anti-CHEK2 antibody WB image
Western blot: (A) K562, (B) Jurkat, and (C) HL-60 whole cell lysates stained with ARG54652 anti-CHEK2 antibody at 1 μg /ml.
