ARG64156
anti-CGI58 / ABHD5 antibody
anti-CGI58 / ABHD5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes CGI58 / ABHD5 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | This antibody is not expected to cross-react with ABHD4. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CGI58 / ABHD5 |
| 抗原物种 | Human |
| 抗原 | C-FPERPDLADQDR |
| 偶联标记 | Un-conjugated |
| 別名 | Lipid droplet-binding protein CGI-58; CGI58; 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; NCIE2; EC 2.3.1.51; CDS; IECN2; Abhydrolase domain-containing protein 5 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8WTS1 Human 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 Swiss-port # Q9DBL9 Mouse 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 |
|---|---|
| 背景介绍 | The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 39 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG64156 anti-CGI58 / ABHD5 antibody WB image
Western Blot: NIH3T3 lysate (35 µg protein in RIPA buffer) stained with ARG64156 anti-CGI58 / ABHD5 antibody at 0.2 µg/ml dilution.
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ARG64156 anti-CGI58 / ABHD5 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64156 anti-CGI58 / ABHD5 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64156 anti-CGI58 / ABHD5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64156 anti-CGI58 / ABHD5 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG64156 anti-CGI58 / ABHD5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64156 anti-CGI58 / ABHD5 antibody at 3.75 µg/ml dilution followed by AP-staining.
