ARG59304
anti-CEP68 antibody
anti-CEP68 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CEP68 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CEP68 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human CEP68. (ELICWLYNVADVTDHGTAARSNLTSLKSSLQLYRQFKKDID) |
| 偶联标记 | Un-conjugated |
| 別名 | Cep68; Centrosomal protein of 68 kDa; KIAA0582 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CEP68 |
| 全名 | centrosomal protein 68kDa |
| 细胞定位 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localizes to thin fibers protruding away from the proximal ends of the two centrioles. Dissociates from interphase centrosomes at the onset of mitosis. [UniProt] |
| 预测分子量 | 81 kDa |
| 翻译后修饰 | Phosphorylation by PLK1 is required for binding to BTRC in prometaphase (PubMed:25503564). Phosphorylated directly or indirectly by NEK2 (PubMed:24554434). NEK2-mediated phosphorylation promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Ubiquitinated and targeted for proteasomal degradation in early mitosis by the SCF(BTRC) and/or SCF(FBXW11) E3 ubiquitin-protein ligase complexes (PubMed:25704143, PubMed:25503564). Degradation is complete by prometaphase and is required for removal of CDK5RAP2 from the peripheral pericentriolar material and subsequent centriole separation (PubMed:25503564). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59304 anti-CEP68 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, COLO-320, SK-OV-3, Jurkat, Rat heart and Mouse heart lysates stained with ARG59304 anti-CEP68 antibody at 0.5 µg/ml, overnight at 4°C.
