ARG66605

anti-CEP135 antibody

anti-CEP135 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes CEP135
反应物种 Hu, Ms
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CEP135
抗原物种 Human
抗原 Synthetic peptide within aa. 1050-1130 of Human CEP135.
偶联标记 Un-conjugated
別名 Centrosomal protein of 135 kDa; KIAA0635; MCPH8; CEP4; Cep135; Centrosomal protein 4

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
IHC-P1:100 - 1:300
WB1:500 - 1:2000
应用说明 IHC-P: Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 130 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol and 0.5% BSA
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 381644 Mouse CEP135

GeneID: 9662 Human CEP135

Swiss-port # Q66GS9 Human Centrosomal protein of 135 kDa

Swiss-port # Q6P5D4 Mouse Centrosomal protein of 135 kDa

基因名称 CEP135
全名 centrosomal protein 135kDa
背景介绍 This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012]
生物功能 Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. [UniProt]
细胞定位 Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles. [UniProt]
预测分子量 133 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG66605 anti-CEP135 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66605 anti-CEP135 antibody at 1:100 dilution (4°C, overnight). Antigen Retrieval: High-pressure and temperature Tris-EDTA buffer (pH 8.0). Negative control (right) was pre-absorbed by immunogen peptide.

  • ARG66605 anti-CEP135 antibody WB image

    Western blot: Mouse lung, Mouse heart and 293T cell lysates stained with ARG66605 anti-CEP135 antibody at 1:1000 dilution.