ARG45701
anti-CENPB antibody
anti-CENPB antibody for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CENPB |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CENPB |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to C-terminal region of human CENPB. |
| 偶联标记 | Un-conjugated |
| 別名 | CENPB; Centromere Protein B; Major Centromere Autoantigen B; Centromere Protein B, 80kDa; CENP-B; Centromere Protein B (80kD); Centromere Autoantigen B |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 80 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CENPB |
| 全名 | Centromere Protein B |
| 背景介绍 | This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008] |
| 生物功能 | Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. [UniProt] |
| 细胞定位 | Centromere; Chromosome; Nucleus. [UniProt] |
| 预测分子量 | 65 kDa |
| 翻译后修饰 | ADP-ribosylation; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45701 anti-CENPB antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45701 anti-CENPB antibody at 5 μg/ml dilution.
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ARG45701 anti-CENPB antibody WB image
Western blot: MCF-7 and PC-3 stained with ARG45701 anti-CENPB antibody at 0.5 μg/ml dilution.
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ARG45701 anti-CENPB antibody FACS image
Flow Cytometry: A431 stained with ARG45701 anti-CENPB antibody at 1 µg/10^6 cells dilution.
