ARG41997

anti-CDT1 / Dup antibody

anti-CDT1 / Dup antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes CDT1 / Dup
反应物种 Hu
应用 ICC/IF, IHC-P, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CDT1 / Dup
抗原物种 Human
抗原 Synthetic peptide of Human CDT1 / Dup.
偶联标记 Un-conjugated
別名 DUP; DNA replication factor Cdt1; RIS2; Double parked homolog

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 HEK293
实际分子量 ~ 65 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 81620 Human CDT1

Swiss-port # Q9H211 Human DNA replication factor Cdt1

基因名称 CDT1
全名 chromatin licensing and DNA replication factor 1
背景介绍 The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011]
生物功能 Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. [UniProt]
细胞定位 Nucleus. Chromosome, centromere, kinetochore. Note=Transiently localizes to kinetochores during prometaphase and metaphase. [UniProt]
预测分子量 60 kDa
翻译后修饰 Two independent E3 ubiquitin ligase complexes, SCF(SKP2) and the DCX(DTL) complex, mediated CDT1 degradation in S phase. Ubiquitinated by the DCX(DTL) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Phosphorylation at Thr-29 by CDK2 targets CDT1 for ubiquitination by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). The interaction with GMNN protects it against ubiquitination. Deubiquitinated by USP37 (PubMed:27296872).

Phosphorylation by cyclin A-dependent kinases at Thr-29 targets CDT1 for ubiquitynation by SCF(SKP2) E3 ubiquitin ligase and subsequent degradation (PubMed:14993212). Phosphorylated at Thr-29 by MAPK8/JNK1, which blocks replication licensing in response to stress (PubMed:21856198). Binding to GMNN is not affected by phosphorylation. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG41997 anti-CDT1 / Dup antibody WB image

    Western blot: HEK293 cell lysate stained with ARG41997 anti-CDT1 / Dup antibody.