ARG58386
anti-CDK8 antibody
anti-CDK8 antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CDK8 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDK8 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 365-464 of Human CDK8 (NP_001251.1). |
| 偶联标记 | Un-conjugated |
| 別名 | K35; Mediator complex subunit CDK8; Protein kinase K35; EC 2.7.11.23; EC 2.7.11.22; Cell division protein kinase 8; Cyclin-dependent kinase 8; Mediator of RNA polymerase II transcription subunit CDK8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat | ||||||
| 实际分子量 | 56 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDK8 |
| 全名 | cyclin-dependent kinase 8 |
| 背景介绍 | The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 53 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG58386 anti-CDK8 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG58386 anti-CDK8 antibody at 1:1000 dilution.
