ARG62434
anti-CDK2 antibody [2B6]
anti-CDK2 antibody [2B6] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody [2B6] recognizes CDK2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-Fr, IHC-P, IP, WB |
| 特异性 | This antibody reacts with a 33 kD protein known as cdk2 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2B6 |
| 同位型 | IgG2b |
| 靶点名称 | CDK2 |
| 抗原物种 | Human |
| 抗原 | Recombinant full length protein (Human) |
| 偶联标记 | Un-conjugated |
| 別名 | p33 protein kinase; Cell division protein kinase 2; p33(CDK2); CDKN2; EC 2.7.11.22; Cyclin-dependent kinase 2 |
应用说明
| 应用说明 | IHC: 1/25 - 1/50 FACS: 1µg for 106 cells WB: 1/100 - 1/500 IP: 1/100 - 1/500 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS (pH 7.4), 1% BSA and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 1% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDK2 |
| 全名 | cyclin-dependent kinase 2 |
| 背景介绍 | This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
| 生物功能 | Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). [UniProt] |
| 产品亮点 | Related products: Cdk2 antibodies; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A. Nitrosylated after treatment with nitric oxide (DETA-NO). |
客户反馈
Average
anti-CDK2 antibody [2B6]
Application：WB
Sample：Mouse testis
Sample Loading Amount：20 µl
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Average
anti-CDK2 antibody [2B6]
Application：WB
Sample：HeLa
Sample Loading Amount：20 µl
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
克隆号文献
DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells.
Productive replication of human papillomavirus 31 requires DNA repair factor Nbs1.
WB / Human
Interference with ER-α enhances the therapeutic efficacy of the selective CDK inhibitor roscovitine towards ER-positive breast cancer cells.
WB / Human
Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial cells impairs estrogen-induced DNA replication, induces DNA damage response pathways, and promotes apoptosis.
IHC-P / Mouse
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
WB / Mouse
Proteomic analysis of waldenstrom macroglobulinemia.
IHC / Human
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
IHC / Hamster
Overexpression of cyclins D1 and D3 during estrogen-induced breast oncogenesis in female ACI rats.
WB / Rat
