ARG45194
anti-CDK12 antibody
anti-CDK12 antibody for Flow cytometry,ICC/IF,Immunohistochemistry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CDK12 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDK12 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human CDK12. |
| 偶联标记 | Un-conjugated |
| 別名 | CDK12; Cyclin Dependent Kinase 12; CRK7; Cdc2-Related Kinase, Arginine/Serine-Rich; KIAA0904; CRKRS; CRKR; Cell Division Cycle 2-Related Protein Kinase 7; Cell Division Protein Kinase 12; CDC2-Related Protein Kinase 7; Cyclin-Dependent Kinase 12; EC 2.7.11.22; CDC2 Related Protein Kinase 7; EC 2.7.11.23; EC 2.7.11 47; HCDK12; CrkRS |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 205 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDK12 |
| 全名 | Cyclin Dependent Kinase 12 |
| 背景介绍 | Enables RNA polymerase II CTD heptapeptide repeat kinase activity and cyclin binding activity. Involved in several processes, including positive regulation of transcription elongation by RNA polymerase II; protein autophosphorylation; and regulation of MAP kinase activity. Located in nuclear speck. Part of cyclin K-CDK12 complex. Biomarker of gastric adenocarcinoma; hepatocellular carcinoma; and stomach cancer. [provided by Alliance of Genome Resources, Nov 2024] |
| 生物功能 | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 164 kDa |
| 翻译后修饰 | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45194 anti-CDK12 antibody IHC-P image
Immunohistochemistry: Human bladder urothelial carcinoma stained with ARG45194 anti-CDK12 antibody at 2 μg/ml dilution.
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ARG45194 anti-CDK12 antibody ICC/IF image
Immunofluorescence: A549 stained with ARG45194 anti-CDK12 antibody at 5 ug/ml dilution.
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ARG45194 anti-CDK12 antibody WB image
Western blot: Hela, K562, Jurkat, and HepG2 stained with ARG45194 anti-CDK12 antibody at 0.5 μg/ml dilution.
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ARG45194 anti-CDK12 antibody FACS image
Flow Cytometry: A549 stained with ARG45194 anti-CDK12 antibody at 1 µg/10^6 cells dilution.
