ARG10887
anti-CDC27 antibody [AF 3.1]
anti-CDC27 antibody [AF 3.1] for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Mammal,Xenopus laevis
概述
| 产品描述 | Mouse Monoclonal antibody [AF 3.1] recognizes CDC27 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Mamm, Xenopus laevis |
| 应用 | ELISA, FACS, IHC-P, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | AF 3.1 |
| 同位型 | IgG1 |
| 靶点名称 | CDC27 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around 10 aa (C-terminus) of Human Cdc27. |
| 偶联标记 | Un-conjugated |
| 別名 | HNUC; Cell division cycle protein 27 homolog; CDC27 homolog; NUC2; D0S1430E; H-NUC; Anaphase-promoting complex subunit 3; CDC27Hs; APC3; ANAPC3; D17S978E |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | HeLa cells. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by affinity chromatography. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDC27 |
| 全名 | cell division cycle 27 |
| 背景介绍 | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014] |
| 生物功能 | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| 预测分子量 | 92 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. [UniProt] |
