ARG41587
anti-CDC23 antibody
anti-CDC23 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CDC23 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDC23 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 50-150 of Human CDC23 (NP_004652.2). |
| 偶联标记 | Un-conjugated |
| 別名 | CUT23; Cell division cycle protein 23 homolog; ANAPC8; APC8; Anaphase-promoting complex subunit 8; Cyclosome subunit 8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat | ||||||
| 实际分子量 | ~ 63 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8BGZ4 Mouse Cell division cycle protein 23 homolog Swiss-port # Q9UJX2 Human Cell division cycle protein 23 homolog |
|---|---|
| 基因名称 | CDC23 |
| 全名 | cell division cycle 23 |
| 背景介绍 | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| 预测分子量 | 69 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG41587 anti-CDC23 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse lung tissue stained with ARG41587 anti-CDC23 antibody at 1:100 dilution.
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ARG41587 anti-CDC23 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG41587 anti-CDC23 antibody at 1:1000 dilution.
