ARG41845
anti-CDC16 / APC6 antibody
anti-CDC16 / APC6 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CDC16 / APC6 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDC16 / APC6 |
| 抗原物种 | Human |
| 抗原 | An 18 amino acid synthetic peptide within the first 50 amino acids of Human CDC16 / APC6. |
| 偶联标记 | Un-conjugated |
| 別名 | Cell division cycle protein 16 homolog; Cyclosome subunit 6; Anaphase-promoting complex subunit 6; CDC16Hs; CDC16 homolog; APC6; CUT9; ANAPC6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Human liver | ||||||
| 实际分子量 | ~ 72 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q13042 Human Cell division cycle protein 16 homolog |
|---|---|
| 基因名称 | CDC16 |
| 全名 | cell division cycle 16 |
| 背景介绍 | This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. [UniProt] |
| 预测分子量 | 72 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG41845 anti-CDC16 / APC6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG41845 anti-CDC16 / APC6 antibody at 5 µg/ml dilution.
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ARG41845 anti-CDC16 / APC6 antibody WB image
Western blot: Human liver lysate stained with ARG41845 anti-CDC16 / APC6 antibody at 1 and 2 µg/ml dilution.
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ARG41845 anti-CDC16 / APC6 antibody IHC image
Immunohistochemistry: Human liver tissue stained with ARG41845 anti-CDC16 / APC6 antibody at 20 µg/ml dilution.
