ARG65389

anti-CD99 antibody [3B2/TA8]

Datasheet

订购信息

包装

价格 (USD)

50 μg

335

arigo in CHINA:
恩瑞科生物
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0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [3B2/TA8] recognizes CD99
反应物种	Hu
应用	CyTOF®-candidate, FACS
特异性	The clone 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes. HLDA VI.; WS Code T 6T-097, BP 534
宿主	Mouse
克隆	Monoclonal
克隆号	3B2/TA8
同位型	IgG2a
靶点名称	CD99
抗原物种	Human
抗原	Human thymocytes
偶联标记	Un-conjugated
別名	12E7; CD99 antigen; MIC2X; MIC2Y; CD antigen CD99; MSK5X; Protein MIC2; MIC2; T-cell surface glycoprotein E2; HBA71; E2 antigen

应用说明

应用建议

应用	推荐稀释比
CyTOF®-candidate	Assay-dependent
FACS	1 - 4 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from cell culture supernatant by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4267 Human CD99 Swiss-port # P14209 Human CD99 antigen
基因名称	CD99
全名	CD99 molecule
背景介绍	CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq, Mar 2016]
生物功能	CD99 involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. [UniProt]
研究领域	Cancer antibody; Immune System antibody; Signaling Transduction antibody
预测分子量	19 kDa
翻译后修饰	Extensively O-glycosylated.

检测图片 (3) Click the Picture to Zoom In

ARG65389 anti-CD99 antibody [3B2/TA8] FACS image

Flow Cytometry: Human peripheral blood stained with ARG65389 anti-CD99 antibody [3B2/TA8] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65389 anti-CD99 antibody [3B2/TA8] FACS image

Flow Cytometry: Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65389 anti-CD99 antibody [3B2/TA8] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65389 anti-CD99 antibody [3B2/TA8] CyTOF image

CyTOF: Human peripheral blood cells (after Ficoll-Paque separation) stained with ARG65389 anti-CD99 antibody [3B2/TA8] (Eu153). Singlet cells were gated for data analysis.