ARG62948

anti-CD9 antibody [EM-04]

anti-CD9 antibody [EM-04] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Mouse

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody

概述

产品描述

Rat Monoclonal antibody [EM-04] recognizes CD9

反应物种 Ms
应用 FACS, ICC/IF, IP, WB
特异性 The clone EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
宿主 Rat
克隆 Monoclonal
克隆号 EM-04
同位型 IgG1
靶点名称 CD9
抗原物种 Mouse
抗原 Permeabilized murine bone marrow-derived mast cells (BMMC).
偶联标记 Un-conjugated
別名 Leukocyte antigen MIC3; BTCC-1; TSPAN-29; MIC3; Tetraspanin-29; p24; Cell growth-inhibiting gene 2 protein; CD9 antigen; MRP-1; DRAP-27; 5H9 antigen; CD antigen CD9; TSPAN29; Motility-related protein; Tspan-29

应用说明

应用建议
应用 推荐稀释比
FACS4 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
WB1 µg/ml
应用说明 WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by protein A
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12527 Mouse CD9

Swiss-port # P40240 Mouse CD9 antigen

基因名称 Cd9
全名 CD9 antigen
背景介绍 CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
生物功能 Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion (By similarity). Acts as a receptor for PSG17. [UniProt]
产品亮点 Related products:
CD9 antibodies; CD9 ELISA Kits; Anti-Rat IgG secondary antibodies;
Related news:
New antibodies for exosome isolation
研究领域 Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody
预测分子量 25 kDa
翻译后修饰 Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.

检测图片 (2) Click the Picture to Zoom In

  • ARG62948 anti-CD9 antibody [EM-04] WB image

    Western blot: Bone marrow-derived mast cell (BMMC) lysates stained with ARG62948 anti-CD9 antibody [EM-04].

  • ARG62948 anti-CD9 antibody [EM-04] FACS image

    Flow Cytometry: Mouse peritoneal fluid cells stained with ARG62948 anti-CD9 antibody [EM-04], followed by incubation with APC labelled Donkey anti-Rat secondary antibody.