anti-CD81 antibody [Eat2]

anti-CD81 antibody [Eat2] for ELISA,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Mouse,Rat


产品描述 Hamster Monoclonal antibody [Eat2] recognizes CD81
Hamster anti Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006).
反应物种 Ms, Rat
宿主 Hamster
克隆 Monoclonal
克隆号 Eat2
同位型 IgG1
靶点名称 CD81
抗原物种 Mouse
抗原 38C13, murine B cell line.
偶联标记 Un-conjugated
別名 CD antigen CD81; TAPA1; Tspan-28; S5.7; CD81 antigen; Target of the antiproliferative antibody 1; Tetraspanin-28; 26 kDa cell surface protein TAPA-1; CVID6; TSPAN28


应用 推荐稀释比
FACS1:10 - 1:50
WBAssay-dependent (for non-reducing conditions)
应用说明 IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
WB: Clone Eat2 recognizes mouse CD81 under non-reducing conditions.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Purification with Protein G.
缓冲液 PBS and 0.09% Sodium azide
抗菌剂 0.09% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 12520 Mouse CD81

GeneID: 25621 Rat CD81

Swiss-port # P35762 Mouse CD81 antigen

Swiss-port # Q62745 Rat CD81 antigen

基因名称 Cd81
全名 CD81 antigen
背景介绍 The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
生物功能 May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. [UniProt]
产品亮点 Related products:
CD81 antibodies; Anti-Hamster IgG secondary antibodies;
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预测分子量 26 kDa
翻译后修饰 Not glycosylated.