ARG22257
anti-CD74 antibody [PIN.1]
anti-CD74 antibody [PIN.1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Mouse Monoclonal antibody [PIN.1] recognizes CD74 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | Detects ~33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PIN.1 |
| 同位型 | IgG |
| 靶点名称 | CD74 |
| 抗原物种 | Human |
| 抗原 | Human CD74 invariant chain synthetic peptide |
| 偶联标记 | Un-conjugated |
| 別名 | DHLAG; Ii; CD antigen CD74; II; HLA-DR antigens-associated invariant chain; Ia-GAMMA; Ia antigen-associated invariant chain; HLA class II histocompatibility antigen gamma chain; p33; HLADG |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P04233 Human HLA class II histocompatibility antigen gamma chain Swiss-port # P04441 Mouse H-2 class II histocompatibility antigen gamma chain |
|---|---|
| 基因名称 | CD74 |
| 全名 | CD74 molecule, major histocompatibility complex, class II invariant chain |
| 背景介绍 | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011] |
| 生物功能 | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [UniProt] |
| 细胞定位 | Cell membrane, Endoplasmic Reticulum, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Lysosome |
| 预测分子量 | 34 kDa |
| 翻译后修饰 | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
检测图片 (1) Click the Picture to Zoom In
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ARG22257 anti-CD74 antibody [PIN.1] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22257 anti-CD74 antibody [PIN.1] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
