ARG56944
anti-CD73 antibody [2B6]
anti-CD73 antibody [2B6] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2B6] recognizes CD73 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2B6 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | CD73 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 27-252 of Human CD73. |
| 偶联标记 | Un-conjugated |
| 別名 | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NT5E |
| 全名 | 5'-nucleotidase, ecto (CD73) |
| 背景介绍 | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| 生物功能 | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| 预测分子量 | 63 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG56944 anti-CD73 antibody [2B6] FACS image
Flow Cytometry: PBMC cell line stained with ARG56944 anti-CD73 antibody [2B6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56944 anti-CD73 antibody [2B6] WB image
Western blot: 30 µg of Human mesenchymal stem cell lysate stained with ARG56944 anti-CD73 antibody [2B6] at 1:1000.
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ARG56944 anti-CD73 antibody [2B6] FACS image
Flow Cytometry: LNCaP cell line stained with ARG56944 anti-CD73 antibody [2B6] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line ).
