ARG62925
anti-CD72 antibody [3F3]
anti-CD72 antibody [3F3] for Flow cytometry,Immunoprecipitation and Human
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [3F3] recognizes CD72 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IP |
| 特异性 | The clone 3F3 reacts with CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells. HLDA V; WS Code B CD72.5 HLDA VI; WS Code B CD72.1 HLDA VI; WS Code 6 BP 84 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3F3 |
| 同位型 | IgG2b |
| 靶点名称 | CD72 |
| 抗原物种 | Human |
| 抗原 | Normal human lymphocytes from a lymph node. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD72; LYB2; B-cell differentiation antigen CD72; Lyb-2; CD72b |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by precipitation methods and ion exchange chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P21854 Human B-cell differentiation antigen CD72 |
|---|---|
| 基因名称 | CD72 |
| 全名 | CD72 molecule |
| 背景介绍 | CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation. |
| 生物功能 | Plays a role in B-cell proliferation and differentiation. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Phosphorylated upon engagement of the B-cell receptor, probably by LYN or SYK. Phosphorylation at Tyr-7 is important for interaction with PTPN6/SHP-1 (By similarity). |
