ARG41769
anti-CD71 / Transferrin Receptor antibody
anti-CD71 / Transferrin Receptor antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD71 / Transferrin Receptor |
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| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD71 / Transferrin Receptor |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to M1-N198 of Human CD71 / Transferrin Receptor. |
| 偶联标记 | Un-conjugated |
| 別名 | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 95 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TFRC |
| 全名 | transferrin receptor |
| 背景介绍 | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
| 生物功能 | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Transferrin receptor protein 1, serum form: Secreted. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. [UniProt] |
检测图片 (6) Click the Picture to Zoom In
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ARG41769 anti-CD71 / Transferrin Receptor antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG41769 anti-CD71 / Transferrin Receptor antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG41769 anti-CD71 / Transferrin Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41769 anti-CD71 / Transferrin Receptor antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41769 anti-CD71 / Transferrin Receptor antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, Jurkat, Raji, HL-60, K562, HepG2, Human placenta and CEM whole cell lysates stained with ARG41769 anti-CD71 / Transferrin Receptor antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41769 anti-CD71 / Transferrin Receptor antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG41769 anti-CD71 / Transferrin Receptor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41769 anti-CD71 / Transferrin Receptor antibody IHC-Fr image
Immunohistochemistry: Frozen section of Human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41769 anti-CD71 / Transferrin Receptor antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41769 anti-CD71 / Transferrin Receptor antibody FACS image
Flow Cytometry: U87 cells were blocked with 10% normal goat serum and then stained with ARG41769 anti-CD71 / Transferrin Receptor antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
