ARG23975
anti-CD71 / Transferrin Receptor antibody [8D3] (FITC)
anti-CD71 / Transferrin Receptor antibody [8D3] (FITC) for Flow cytometry and Mouse
概述
| 产品描述 | FITC-conjugated Rat Monoclonal antibody [8D3] recognizes CD71 / Transferrin Receptor |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | 8D3 |
| 同位型 | IgG2a |
| 靶点名称 | CD71 / Transferrin Receptor |
| 抗原物种 | Mouse |
| 抗原 | Mouse transformed endothelioma cell line. |
| 偶联标记 | FITC |
| 別名 | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide and 1% BSA. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TFRC |
| 全名 | transferrin receptor |
| 背景介绍 | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
| 生物功能 | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Transferrin receptor protein 1, serum form: Secreted. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23975 anti-CD71 / Transferrin Receptor antibody [8D3] (FITC) FACS image
Flow Cytometry: Cells derived from Mouse bone marrow stained with anti-TER-119 antibody (PE) and ARG23975 anti-CD71 / Transferrin Receptor antibody [8D3] (FITC).
