ARG62915
anti-CD66e / CEA antibody [CB30]
anti-CD66e / CEA antibody [CB30] for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
Cancer antibody; Developmental Biology antibody; Controls and Markers antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Mouse Monoclonal antibody [CB30] recognizes CD66e / CEA |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-Fr, IHC-P, IP |
| 特异性 | The clone CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CB30 |
| 同位型 | IgG1 |
| 靶点名称 | CD66e / CEA |
| 抗原物种 | Human |
| 抗原 | Human carcinoembryonic antigen (CEA; CEACAM5) |
| 偶联标记 | Un-conjugated |
| 別名 | Carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; CD antigen CD66e; Carcinoembryonic antigen; Meconium antigen 100 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Staining technique: Standard ABC technique (DAB+). Pretreatment: 0.1% pepsin in 0.1M HCl for 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-P: Adenocarcinoma of colon |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P06731 Human Carcinoembryonic antigen-related cell adhesion molecule 5 |
|---|---|
| 基因名称 | CEACAM5 |
| 全名 | carcinoembryonic antigen-related cell adhesion molecule 5 |
| 背景介绍 | The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting._x000D_ |
| 生物功能 | Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. [UniProt] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Controls and Markers antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. |
检测图片 (2) Click the Picture to Zoom In
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ARG62915 anti-CD66e / CEA antibody [CB30] IHC-P image
Immunohistochemistry: Colorectal carcinoma (paraffin-embedded sections) stained with ARG62915 anti-CD66e / CEA antibody [CB30].
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ARG62915 anti-CD66e / CEA antibody [CB30] IHC-P image
Immunohistochemistry: Colorectal carcinoma (paraffin-embedded sections) stained with ARG62915 anti-CD66e / CEA antibody [CB30].
克隆号文献
Rapidly derived colorectal cancer cultures recapitulate parental cancer characteristics and enable personalized therapeutic assays.
Establishment of human ultra-low passage colorectal cancer cell lines using spheroids from fresh surgical specimens suitable for in vitro and in vivo studies.
FACS /
