ARG23283
anti-CD63 antibody [AD1] (FITC)
anti-CD63 antibody [AD1] (FITC) for Flow cytometry and Rat
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [AD1] recognizes CD63 Mouse anti Rat CD63 antibody, clone AD1 specifically recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, which is found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1. Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release (Kitani et al. 1991). |
|---|---|
| 反应物种 | Rat |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | AD1 |
| 同位型 | IgG1 |
| 靶点名称 | CD63 |
| 抗原物种 | Rat |
| 抗原 | RBL-2H3 Rat basophilic leukaemia cell line. |
| 偶联标记 | FITC |
| 別名 | Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide and 1% BSA. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD63 |
| 全名 | CD63 molecule |
| 背景介绍 | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| 生物功能 | Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23283 anti-CD63 antibody [AD1] (FITC) FACS image
Flow Cytometry: Rat peripheral blood platelets stained with ARG23283 anti-CD63 antibody [AD1] (FITC).
