ARG23112
anti-CD58 antibody [BRIC5]
anti-CD58 antibody [BRIC5] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [BRIC5] recognizes CD58 Mouse anti Human CD58 antibody, clone BRIC5 recognizes human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgoba et al. 1989).CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. BRIC5 inhibits T cell rosetting. |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | BRIC5 |
| 同位型 | IgG2a |
| 靶点名称 | CD58 |
| 抗原物种 | Human |
| 抗原 | Human erythrocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD58; LFA3; ag3; Lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3; Ag3; LFA-3 |
应用说明
| 应用建议 |
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| 应用说明 | WB: This antibody binds a broadly migrating component of 40-65 kDa under non-reducing conditions. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | Tissue Culture Supernatant and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P19256 Human Lymphocyte function-associated antigen 3 |
|---|---|
| 基因名称 | CD58 |
| 全名 | CD58 molecule |
| 背景介绍 | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009] |
| 生物功能 | Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. [UniProt] |
| 预测分子量 | 28 kDa |
