ARG23088
anti-CD49f / Integrin alpha 6 antibody [NKI-GoH3] (PE)
anti-CD49f / Integrin alpha 6 antibody [NKI-GoH3] (PE) for Flow cytometry and Human,Mouse,Dog,Pig,Sheep
概述
| 产品描述 | PE-conjugated Rat Monoclonal antibody [NKI-GoH3] recognizes CD49f / Integrin alpha 6 Rat anti Human CD49f antibody, clone NKI-GoH3 recognizes CD49f, also known as the VLA-6 alpha chain, CD49f is a 1107 amino acid ~120 kDa cell surface glycoprotein that forms distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex expressed on various human epithelial cells. Rat anti Human CD49f antibody, clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Rat anti Human CD49f antibody, clone NKI-GoH3 blocks the binding of cells to the E8 fragment of laminin (Sonnenberg et al. 1998). |
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| 反应物种 | Hu, Ms, Dog, Pig, Sheep |
| 应用 | FACS |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | NKI-GoH3 |
| 同位型 | IgG2a |
| 靶点名称 | CD49f / Integrin alpha 6 |
| 抗原物种 | Human |
| 抗原 | BALB/c Mouse mammary tumour cells. |
| 偶联标记 | PE |
| 別名 | CD49 antigen-like family member F; ITGA6B; Alpha6p; VLA-6; CD antigen CD49f; CD49f; Integrin alpha-6 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 106 platelets in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | ITGA6 |
| 全名 | integrin, alpha 6 |
| 背景介绍 | The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. [UniProt] |
| 预测分子量 | 127 kDa |
| 翻译后修饰 | Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand. In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro. Palmitoylation by DHHC3 enhances stability and cell surface expression. |
检测图片 (1) Click the Picture to Zoom In
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ARG23088 anti-CD49f / Integrin alpha 6 antibody [NKI-GoH3] (PE) FACS image
Flow Cytometry: Figure A. Alexa Fluor488 conjugated Mouse anti Human CD3 and PE-conjugated Rat IgG2a isotype control. Figure B. Alexa Fluor488 conjugated Mouse anti Human CD3 and ARG23088 anti-CD49f / Integrin alpha 6 antibody [NKI-GoH3] (PE). All experiments performed on Human peripheral blood lymphocytes.
