ARG55664
anti-CD49f / Integrin alpha 6 antibody [129CT49.6.5]
anti-CD49f / Integrin alpha 6 antibody [129CT49.6.5] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CD49f / Integrin alpha 6 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 129CT49.6.5 |
| 同位型 | IgG1 |
| 靶点名称 | CD49f / Integrin alpha 6 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human Integrin alpha 6. |
| 偶联标记 | Un-conjugated |
| 別名 | CD49 antigen-like family member F; ITGA6B; Alpha6p; VLA-6; CD antigen CD49f; CD49f; Integrin alpha-6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGA6 |
| 全名 | integrin, alpha 6 |
| 背景介绍 | The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein Cell membrane; Lipid-anchor |
| 预测分子量 | 127 kDa |
| 翻译后修饰 | Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand. In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro. Palmitoylation by DHHC3 enhances stability and cell surface expression. |
检测图片 (3) Click the Picture to Zoom In
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ARG55664 anti-CD49f / Integrin alpha 6 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human skin carcinoma stained with ARG55664 anti-CD49f / Integrin alpha 6 antibody.
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ARG55664 anti-CD49f / Integrin alpha 6 antibody WB image
Western blot: 35 µg of HepG2 cell lysate stained with ARG55664 anti-CD49f / Integrin alpha 6 antibody.
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ARG55664 anti-CD49f / Integrin alpha 6 antibody FACS image
Flow Cytometry: HepG2 cells stained with ARG55664 anti-CD49f / Integrin alpha 6 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with PE labelled secondary antibody.
