ARG23116
anti-CD49d / LPAM1 / Integrin alpha 4 antibody [44H6]
anti-CD49d / LPAM1 / Integrin alpha 4 antibody [44H6] for Flow cytometry,IHC-Frozen sections and Human
概述
| 产品描述 | Mouse Monoclonal antibody [44H6] recognizes CD49d / LPAM1 / Integrin alpha 4 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-Fr |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 44H6 |
| 同位型 | IgG1 |
| 靶点名称 | CD49d / LPAM1 / Integrin alpha 4 |
| 抗原物种 | Human |
| 抗原 | HOON pre-B leukaemia cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | CD49 antigen-like family member D; VLA-4 subunit alpha; CD49D; Integrin alpha-IV; CD antigen CD49d; Integrin alpha-4; IA4 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-Fr: Overnight incubation with clone 44H6 is recommended for optimal results. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 100 µl of human whole peripheral blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by ion exchange chromatography. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGA4 |
| 全名 | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| 背景介绍 | The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. [provided by RefSeq, Jul 2008] |
| 生物功能 | Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. [UniProt] |
| 预测分子量 | 115 kDa |
| 翻译后修饰 | Phosphorylation on Ser-1027 inhibits PXN binding. |
检测图片 (1) Click the Picture to Zoom In
-
ARG23116 anti-CD49d / LPAM1 / Integrin alpha 4 antibody [44H6] FACS image
Flow Cytometry: Human peripheral blood lymphocytes stained with ARG23116 anti-CD49d / LPAM1 / Integrin alpha 4 antibody [44H6].
