ARG52786
anti-CD45 antibody [SPM496]
anti-CD45 antibody [SPM496] for IHC-Formalin-fixed paraffin-embedded sections and Human
Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
概述
| 产品描述 | Mouse Monoclonal antibody [SPM496] recognizes CD45/T200/LCA |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SPM496 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD45 |
| 抗原物种 | Human |
| 抗原 | Stimulated human leucocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||
| 阳性对照 | Tonsil |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C |
|---|---|
| 背景介绍 | CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| 生物功能 | CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt] |
| 细胞定位 | Membrane |
| 产品亮点 | Related products: CD45 antibodies; CD45 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody |
| 预测分子量 | 147 kDa |
| 翻译后修饰 | Heavily N- and O-glycosylated. |
检测图片 (10) Click the Picture to Zoom In
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Cervical Squamous Cell Carcinoma stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Tonsil stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Colon stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Endometrial Adenocarcinoma stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Esophagus stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human HK Lymphoma stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Lung stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Reactive Lymph Node stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Stomach Adenocarcinoma stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
-
ARG52786 anti-CD45/T200/LCA antibody [SPM496] IHC-P image
Immunohistochemistry: Human Thymus stained with CD45/T200/LCA antibody [SPM496] (ARG52786)
