ARG23038

anti-CD45 antibody [OX-1] (PE)

anti-CD45 antibody [OX-1] (PE) for Flow cytometry and Rat

Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody

概述

产品描述 PE-conjugated Mouse Monoclonal antibody [OX-1] recognizes CD45
Mouse anti Rat CD45 antibody, clone OX-1 recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.This product is routinely tested in flow cytometry on rat splenocytes
反应物种 Rat
应用 FACS
宿主 Mouse
克隆 Monoclonal
克隆号 OX-1
同位型 IgG1
靶点名称 CD45
抗原物种 Rat
抗原 Rat thymocyte membrane glycoproteins.
偶联标记 PE
別名 LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220

应用说明

应用建议
应用 推荐稀释比
FACSNeat
应用说明 FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Unpurified.
缓冲液 PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose
抗菌剂 0.09% Sodium azide
稳定剂 1% BSA and 5% Sucrose
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 24699 Rat PTPRC

Swiss-port # P04157 Rat Receptor-type tyrosine-protein phosphatase C

基因名称 Ptprc
全名 protein tyrosine phosphatase, receptor type, C
背景介绍 CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
生物功能 CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt]
研究领域 Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody; B Cell Marker antibody
预测分子量 147 kDa
翻译后修饰 Heavily N- and O-glycosylated.

检测图片 (2) Click the Picture to Zoom In

  • ARG23038 anti-CD45 antibody [OX-1] (PE) FACS image

    Flow Cytometry: Rat spleen cells stained with ARG23038 anti-CD45 antibody [OX-1] (PE).

  • ARG23038 anti-CD45 antibody [OX-1] (PE) FACS image

    Flow Cytometry: Figure A. ARG23038 anti-CD45 antibody [OX-1] (PE) and FITC conjugated mouse IgG2a isotype control. Figure B. ARG23038 anti-CD45 antibody [OX-1] (PE) and FITC conjugated mouse anti rat CD26. All experiments performed on red cell lysed rat blood gated on mononuclear cells.